@fishy_fiz
I don't know of any programs that didn't require loading Kickstart to boot, but some programs kicked Kickstart out and used the 256K WOM for themselves.
The one that comes immediately to mind is the A-Max Macintosh emulator. A 512K A1000 could have 512K free in MacOS, with the Mac ROMs loaded into the Kickstart area, plus a RAM disk as well if you were using a small ROM image.
There were a few games that I remember went through what seemed like a two-level boot process. Rebooting the machine after playing the game required re-loading Kickstart. I don't know if the games were doing that on purpose to use the KS memory or if they were accidentally corrupting Kickstart.