@FlashLab:
If it a stock A4000 floppy cable, the internal floppy drive should be connected to the middle connector of the floppy cable and the floppy cable itself should be connected to the motherboard with the connector that is farthest apart from the other two connectors (the end connector which doesnt have a bit of the cable twisted just before the connector). Make sure that is the case.
On an A4000, DF2: is the first connected external floppy drive as DF0: and DF1: are reserved for internal drives.
Try some other disks too, so you are sure it isnt something with a wierd bootblock or similar crashing the machine.
Btw, the A4000 works fine without fastmem, albeit not overly usable and very slow. You need the doublesided 2MB chipmem simm to be present though (the one closest to the Zorro daughterboard).
/Patrik