As Tomas said, create an iso and then boot the system with an emergency disk that mounts the CF as boot disk instead of the cdrom. For that purpose you can happily use the emergency disk that amije (he's a member here and of course in amigahellas.gr forum) has created.
http://files.amigahellas.gr/data/Utilities/cf-boot-disk.lhaI had the same question to do regarding OS4. Could someone avoid cdrom usage and use a CF instead or even a USB stick? Maybe this requires more tweaking.