No, because this signal is a hardware level interrupt. On the A3000 fast slot, which is where it's often missing from, it's usually used for SCSI controller interrupts on a CPU board. You don't have software control over this interrupt.
However, it's only a quick test with an Ohmmeter to confirm if you need to modify the board or not.
There's a guide I wrote on it
here, refer to the image at the bottom of the page on
testing.