Quake 1 claimed it did but didn't. I played it on a 486/25SX*. A bit on the jerky side, but it worked :-)
* - the 486 SX range were the cheaparse CPUs without an FPU. The DX range had a built in FPU.
FPUs are very nice. Buy accelerators with them if you can. I've seen a few apps I've installed specify 040/FPU as an option.