AF6 is not an OS, it is a software package. It comes with a ready-to-run AmigaOS. AmigaOS only runs on Amiga hardware, so you need an emulator like UAE to run it on PC hardware. UAE is contained in the AF6 package, too.
If you boot from the CD, the first OS started is Knoppix. It is an OS which runs on PC hardware. Knoppix then starts UAE and UAE runs AmigaOS. All of this is contained on the AF6 CD, no other package needed.
If you have Windows installed on your PC, insert the AF6 CD and the launcher will pop up. You can then choose different configurations for WinUAE and WinUAE will load and run AmigaOS. WinUAE and AmigaOS are contained on the CD. The CD contains AmigaOS1.3 on ADF files and a mix of AmigaOS 3.1 and 3.9 in directories used as harddrives in WinUAE.
Concluding the CD contains almost all you need. There are even some applications on it (PPaint, DOpus, TurboText ...)
Bye,
Thomas