Well, you could try it without. I tried it with WinUAE and it does indeed boot into Workbench. However, the background pictures does not load and there is only 78kb of memory left. You can't even run HDToolbox to partition your HDD.
Edit: no, that's not true. If you close AmiDock, thre are more than 400kb left and HDToolbox runs fine :-)
Bye,
Thomas