Hmm, you may be correct, now that I think about it, I've never actually tested it. Memory a bit fuzzy on that front.
Doesn't really matter though as why would you spend the extra money to buy a 2M one when either way you'd get no benefit over a 1M one if you're just doing a drop-in replace?
No it didn't work on my B2000 Rev6 1Mb CHIP RAM configured machine. I tried to put 8375 Super Fat Agnus (from A500Plus) just for fun, but it didn't even fired up...