Uhm... well...
You plug in the HD into your PC that runs 2000 or XP.
That all that is needed for WinUAE to recognize the HD.
If you want to be able to play with it with HDToolbox, I think you would have to use the uaehf.device (don't remember if this was to be found internally when running WinUAE or if you would have to install it under AOS under WinUAE, think it was internally emulated though).