Looking at the picture of the IDE-Fix adapter (see below) ... Pin 1 (the one that red stripe on cable connects to) is on the bottom edge of the adaptor, denoted by the 1 printed on the board.

I presume, that in your second question, you refer to the 5 pin connector on the edges of both cards? According to the makers, if you plug the cable in the wrong way around then the software will nag you to swap it to the correct orientation ... nothing will break or go bang as the software will do a diagnostic upon startup and report any installation or adaptor faults.
