I used a GVP HD8 on my 1000 and SKick'ed to Kickstart 3.1. I cannot recall if the SCSI boot ROM allowed the system to boot directly from the hard drive or if I had to leave a Kickstart 1.3 disk in the floppy drive. I would expect it was the latter.
Either way, I was able to run 3.1 on my mostly-stock 1000.