If you have an A500 with Kickstart, you aren't seeing a Kickstart screen - correct? You're seeing a screen asking for Workbench?
I'd guess either all your drives are bad, the ribbon cables were not connected properly, Gary or CIA chip went bad. More likely a CIA chip though since they're also responsible for the joy/mouse ports and people LOVE to plug and unplug their mice and joysticks when the computer is on. Quite common for the CIA's to blow actually. Swap them with each other and see what happens. There's two of 'em labeled 8520...