Yeah, since it worked last time you used it 10 years ago and depending where you
stored it - I wouldn't be surprised if the drive has become magnetised, dirty or misaligned.
Maybe try cleaning it with a floppy drive cleaner or if you don't have one, a Q-tip and
(preferably) tape head cleaner and failing that, rubbing alcohol.
Oh and may we assume that there's nothing on the side expansion bus contributing
to this poor thing not booting?
After writing a new KS disk and it STILL doesn't work, you could always swap the
500's floppy with the A1000's as a last ditch shotgun attempt at troubleshooting.
They say not knowing is "half" the battle... lol