Yes, AMOS (The Creator and Pro) aren't compatible with accelerator cards and some other extra's.
Francois Lionet made it compatible with OCS and ECS in mind. Unless you have the various AGA related 3rd-party extensions for AMOS Pro you can't do anything on AGA either (other than OCS/ECS stuff).
I have most versions of AMOS, but with my A600's down I am forced to work under emulation (WinUAE 1.4.0) on my laptop.
Regards,
Lonewolf10