Installed 3.1.4.1 on my Amiga 600 this afternoon. The machine was working fine with 2.04 but after the installation of the 3.1.4 ROM I couldn't get it to boot. Only solution to boot from Hard Drive now is to use the early startup to disable the CC0: drive and to disable the CPU caches.
Is there a fix coming in AmigaOS 3.2...seems to be a common problem.