The temperature test is a good trick to start with but it doesn't work all the time. (It's surprisingly good though - I've repaired a couple of machines w/o any schematics that way!)
If everything else fails, there's no hint of bad contact on Agnus' socket, no bad solder points on the RAMs or the buffers (U34/35 - thermal?) you'll be left with desoldering each RAM chip and test it elsewhere...
Dunno if it's been covered before: doing the 1 MB chipram mod doesn't require you to have the RAM expansion in place all the time. If it's not present there's simply no difference to an unmodded board.
Then you must do 40 unsolderings and 40 solderings (20pins on both sides of mb=40)!
Don't really get that - you desolder und resolder
from both sides?
Actually, 20 pins is pretty fast if the vias aren't too tight and you've got a bit of practice - once you know which one's wrong.