Will an Amiga 2000 work without the battery on the motherboard ?
Yes. The only thing you lose is the battery backup feature of the real-time clock (RTC). (Each time you boot the Amiga it'll think it's 1978 until you set the clock by some means.)
Does any one know what the Tick signal (Pin 14 on the Powersupply connecter) should be ?
Don't know. I think it might depend on the region... I never understood the power supply well enough to tinker with it much. ;-) I did find this link in google's cache, though.
GoogleCache link