This is just a thought but have you done the INT line fix? I seem to remember doing this in my 3000 a while back when I installed an A3640 card. I installed a Rev.11 Superbuster but left the original Dmac 2 and Ramsey 4 revisions. I had no problem using a GVP Spectrum card until I eventually upgraded with a Cyberstorm/Cybervision combo. I don't know what A3000 m/b revision I have though (too much of a pain to rip it all apart to check ..sorry)
One last question: How much system memory and are you using an accelerator at all?