Probably. If you can find out what filesystem they used for the CDTV/A570. Joliet perhaps (just guessing, I know very little about CD filesystems)?
I read somewhere in an old computer mag that the A570 CDs would "hold as much as 550MB of data", so try staying below that limit.
If you're going to use and IDE CD-ROM or some SCSI version together with a SCSI hard drive expansion you'd probably need at least OS2.04 to access the CD.
