The docs don't list any OS requirements as you've probably noticed, but in various sections they detail:
"Starting with AmigaDOS 3.0 ......" suggesting some slightly different behaviour from previous OS versions, i.e. OS2.x. So perhaps operation could be possible under 2.x??
Never tried it on anything less than OS3.1 myself, though I can confirm MovieShop 4.3 runs fine under OS3.9 + BB2.
A bit off topic, but here's some good info on additional operators, if you have them:
Video Works.