Hi amiga1260,
You may have a bad disk drive ribbon cable. Do NOT put any creases in that ribbon cable! If there are any creases in the disk drive ribbon cable, replace it with a new one. Also, make sure that you are NOT having ANY problems with your SCSI drives. Any problems with the SCSI system can cause the Amiga to get confused. This will then cause read / write failures from the 3.5" disk drives. You might also want to try replacing Paula. Not only is she responsible for producing the incredible stereo sounds and music that we hear, she also contains the microdisk controller. Be careful though, Paula is easily damaged by static electricity. :-D