Gaah, you're killing me now, man. You just need to copy the file from where ever it is on the floppy disk, into the C directory on your hard drive. Have you never used DOS before?

Like if the file is in the directory "Tools", you'd type:
copy df0:tools/acatune to SYS:C
Or if the file is in a directory called "Utilities", you'd type:
copy df0:utilities/acatune to SYS:C
Or if the file is on a floppy disk in an external drive, you'd replace df0: with df1: or df2: or whatever your floppy disk is. Are you familiar with commands like CD, Dir, List, etc.? You're making me feel like an old fogey now, for using dos commands, haha.

I don't know where the file is on the floppy disk, but it's there somewhere, probably in some subdirectory. You need to get it from there, into the C directory on your boot partition so you can add it to your Startup-Sequence.