Looks like it might be the hard drive. I am getting these issues when I run off the IDE drive. If I run the software off the SCSI drive (running with a 2091 card, which is really slow) the framestore and chromafx problems I was having go away.
I looked in the HDToolbox and didn't see any settings for Max Transfer. I looked under the partition drive area advanced tab too. Is this option available in 3.1? (I am used to the old 1.3 Amigas)
That definitely sounds like a MaxTransfer issue. Click the Advanced checkbox in HDToolbox, then the Change... button. It'll be in the lower left of the window that appears.