It should work, if you have a SCSI controller. I had a CRAPPY 1X SCSI CDRom attached to my old A500, via a Trumpcard SCSI controller's daisy chain.
Of course, I haven't had an a500, since 1996. ^_~ By then, I had upgrated to my a2000 and only a year later, got my a3000, before it took a dump and died. *frowns*