OK well on OS3 you've got Irmen de Jong's original 2.0 port which is pretty complete and robust but obviously stuck at Python 2.0 so it's unlikely to work with much recent existing code and packages even those for Python 2.7. Then there's my own update of 2.0 to 2.3.3 which was half baked but might be of some use. I had 2.4 running on my Amiga of 2005 but not in a stable enough form to release, I was attempting to merge Irmen's Amiga add-ons into 2.4 whilst also switching the toolchain to GCC and clib2 instead of SAS/C and Irmen's own POSIX functions library. Alternatively I was also looking at using VBCC and it's PosixLib. But in the end I switched to porting it to Symbian OS instead.
Around this time the OS4 port begun, and the current OS4 version is 2.5.6, which is about 4 years behind the current 2.7 baseline. But I imagine it can be used for most things without much difficulty. It supports libdl stuff so a lot of addon packages that have native dependencies can be used especially PIL. It doesn't have all the neato Amiga integration tricks Irmen's version had though. Some random person has also released a build of Python 2.4 for OS3 on Aminet, apparently built using the OS4 sources, and I would really like to know where they got them, because all of my approaches to collaborate on backporting it to OS3 over the years were aggressively rebuffed, then and now.
As for Python 3 ports, there's a guy who's done a recent port of that as a pure POSIX version, no Amiga-isation, on OS4 using GCC and his own fork of clib2. I've heard rumours of other Python 3 ports too. There's a thread about that on amigans. He hasn't released it yet.
Currently I'm discussing a collab with a bunch of guys about doing a new set of Python2 and Python3 ports for OS3, OS4, MorphOS and AROS sharing a mostly common codebase.